A simple and economical first order derivative spectrophotometric method and dual wavelength method were developed for simultaneous estimation of ketorolac tromethamine (KET) and phenylephrine (PHE) in its synthetic mixture. The wavelengths selected for first order spectrophotometric method were 331 nm for KET (zero crossing point for PHE) and 231 nm for PHE (zero crossing point for KET). For the Dual wavelength method 315 and 341.8 nm for KET and 266.2 and 278.2 nm for PHE wavelengths were selected. The linearity range was found to be 6-14 µg/ml for KET and 24-56 µg/ml for PHE for first order derivative and dual wavelength method. The co-relation coefficient was found to be 0.9991 and 0.9994 and the assay value was found to be 98.9% and 99.7% for first order spectroscopic method. For dual wavelength method the co-relation coefficient was found to be 0.9993 and 0.9995 and the assay value was found to be 98.5 % and 99.72% respectively for KET and PHE. All the methods were found to be simple, accurate and reproducible. The methods were validated as per ICH Guidelines.
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